Journal article
Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum
SC Oehring, BJ Woodcroft, S Moes, J Wetzel, O Dietz, A Pulfer, C Dekiwadia, P Maeser, C Flueck, K Witmer, NMB Brancucci, I Niederwieser, P Jenoe, SA Ralph, TS Voss
Genome Biology | Published : 2012
Abstract
Background: The post-genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due to the large evolutionary distance to model eukaryotes, however, we lack a profound understanding of many processes in Plasmodium biology. One example is the cell nucleus, which controls the parasite genome in a development- and cell cycle-specific manner through mostly unknown mechanisms. To study this important organelle in detail, we conducted an integrative analysis of the P. falciparum nuclear proteome. Results: We combined high accuracy mass spectrometry and bioinformatic approaches to present for the first time an experimentally determined core nuclear pro..
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Awarded by University of Melbourne
Funding Acknowledgements
The authors would like to thank Terry Speed and James Bailey for helpful comments. We are grateful to Tim-Wolf Gilberger, Mike Duffy, Michael Terns, and Geoff McFadden for providing antibodies. BJW is funded by a University of Melbourne MRS scholarship. SAR is funded by an Australian Research Council Future Fellowship (FT0990350). NMBB received a Boehringer Ingelheim PhD fellowship. This work was supported by the Swiss National Science Foundation (PP00A-110835; PP00P3_130203), the Novartis Foundation for Medicine and Biology (08C46), the Emilia-Guggenheim-Schnurr Foundation, and the Rudolf Geigy Foundation. The funders had no role in study design, data collection, analysis, and interpretation, the decision to publish, or preparation of the manuscript.